First demonstration of an inhibitory activity of milk proteins against human immunodeficiency virus-1 reverse transcriptase and the effect of succinylation

摘要

A variety of milk proteins including lactoferrin, angiogenin-1, alpha -lactalbumin, beta -lactoglobulin, lactoperoxidase, casein and the novel whey proteins lactogenin and glycolactin were tested for inhibitory activity toward human immunodeficiency virus-1 reverse transcriptase (HIV-1 RT), alpha -glucosidase, beta -glucosidase and beta -glucuronidase. Lactoferrin exerted the most potent inhibitory action with an IC50 of about 6 muM. Lactoperoxidase, lactogenin, angiogenin-1 and glycolactin inhibited HIV-1 RT activity with decreasing potencies. beta -Lactoglobulin, alpha -lactalbumin and casein displayed little or no inhibitory effect. Succinylation with succinic anhydride augmented the inhibitory effect of glycolactin, beta -lactoglobulin, alpha -lactalbumin, casein and human lactoferrin. The inhibitory effect of the various milk proteins on the activities of alpha -glucosidase, beta -glucosidase and beta -glucuronidase was meager. Succinylation tended to increase the alpha -glucosidase-inhibitory effect of milli. proteins but neither their beta -glucosidase-inhibitory nor beta -glucuronidase-inhibitory effect was affected. (C) 2000 Elsevier Science Inc. All rights reserved. [References: 29]