Complementary Selectivities of Synergi~(TM) Polar-Functionalized HPLC Columns for Nucleobases and Nucleosides

摘要

A variety of high performance liquid chromatography (HPLC) methods have been developed for the analysis of pyrimi-dine-purine bases and the corresponding 2'-deoxynucleosides, the basic building blocks of nucleic acids. These include reversed phase (RP-HPLC), ion exchange (IEX), and normal phase chromatography (1,2). These molecules are highly polar and hence their retention on traditional C18 and other alkylsilylated phases under RP-HPLC conditions is problematic. Ion-pairing agents are commonly used to enhance retention for highly polar compounds; however, the use of ion-pair reagents adds a complexity to the mobile phase and can result in lengthy column equilibration. In this note, the retention characteristics of these biologically important probes on three polar, reversed-phase columns: Synergi? Hydro-RP (polar-end-capped C18), Synergi? Polar-RP? (ether functionalized phenyl, polar-endcapped), and Synergi? Fusion-RP (a polar-embedded C18 phase) are investigated in the absence of ion-pairing agents.