Countercurrent Chromatography

摘要

Countercurrent chromatography (CCC) is experiencing a renaissance and offers a variety of benefits for preparative chromatography. This technique is useful for the isolation of natural products and can eliminate some of the problems associated with solid-phase adsorbents. Countercurrent chromatography (CCC) is basically the liquid-liquid partition of a sample between two immiscible liquid phases. This is the same separation principle as separating two mixtures using a simple separating funnel experiment. The technique developed from countercurrent distribution, which was investigated in the 1940s to provide separations without a solid chromatographic support. The term "countercurrent chromatography", although generally accepted nowadays, is in fact a misnomer because most systems involve the passage of one phase (the mobile phase) of a biphasic solvent system through a second non-mobile (stationary phase) component of the solvent system.