Vitamin E reduces plasma low density lipoprotein cholesterol, LDL oxidation, and early aortic atherosclerosis compared with black tea in hypercholesterolemic hamsters.

摘要

80 male F1B Golden Syrian hamsters, 7 weeks of age, were divided into 4 groups of 20. The hamsters were fed a semi-purified hypercholesterolaemic diet containing 12% coconut oil, 3% sunflower oil, and 0.2% cholesterol (w/w) (control (C)), and eitherC + 0.625% brewed black tea (w/w) (low tea (LT)), C + 1.25% brewed black tea (w/w) (high tea (HT)), or C + 0.044% tocopherol acetate (w/w) (vitamin E (VE)) for 10 weeks. The hamsters fed the VE diet compared to the C, LT, and HT diets had significantlylower plasma LDL-cholesterol concentrations (-18%, P<0.007; -17%, P<0.02; and -24%, P<0.0001; respectively). Aortic fatty streak area was reduced in the VE diet compared to the C (-36%, P<0.04) and LT (-45%, P<0.01) diets. Lag phase of conjugated diene production was greater in the VE diet compared to the C, LT, and HT diets (41%, P<0.0004; 40%, P<0.0004; and 39%, P<0.0008; respectively). Rate of conjugated diene production was reduced in the VE diet compared to the C, LT, and HT diets (-63%, P<0.002; -57%, P<0.005; and -59%, P<0.02; respectively). Also, maximum number of conjugated dienes produced was lower in the VE diet compared to the C(-14%, P<0.05) and LT (-22%, P<0.006) diets. The VE diet had greater concentrations of LDL alpha-tocopherol compared to the C, LT, and HT diets (69%, P<0.0001; 71%, P<0.0001; and 65%, P<0.0001; respectively). The primary findings of the present study were that dietary vitamin E supplementation reduces plasma LDL-cholesterol concentrations, LDL oxidation and early atherosclerosis compared to black tea consumption in the hypercholesterolaemic hamster and that the antioxidant actions of vitamin E is through the incorporation of vitamin E into the LDL molecule.