Nucleosides and oligonucleotides with diynyl side chains: the huisgen-sharpless cycloaddition 'click reaction' performed on DNA and their constituents.

摘要

Phosphoramidite building blocks related to the four canonical DNA constituents and oligonucleotides with octadiynyl side chains (5b-8b) or dipropargyl ether residues (5c) were synthesized. Nucleosides and oligonucleotides were functionalized at the terminal triple bonds employing the Huisgen-Sharpless cycloaddition "click reaction."