Random amplified hybridization microsatellites (RAHM): isolation of a new class of microsatellite-containing DMA clones

摘要

Microsatellite repeats are widely present in eukaryotic genomes (1,2) and are interspersed throughout the genome DNA. Microsatellite markers have been developed for a variety of organisms (3,4), including plants (5-7). The regions flanking microsatellite sequences can be amplified in a PCR reaction using microsalellite primers, thus providing codominant sequence tagged sites (STS) markers. However, efforts to develop such molecular markers are quite laborious, requiring library construction, screening, and sequencing of clones. Several groups have developed alternative methods for detection (8) and isolation of flanking sequences (9,10) using PCR. Random amplified polymorphic DNA (RAPD) markers are easy to develop and less expensive to assay, but generally are genetically dominant and have a low degree of polymorphism.