DELETIONS AT SHORT DIRECT REPEATS AND BASE SUBSTITUTIONS ARE CHARACTERISTIC MUTATIONS FOR BLEOMYCIN-INDUCED DOUBLE- AND SINGLE-STRAND BREAKS, RESPECTIVELY, IN A HUMAN SHUTTLE VECTOR SYSTEM

摘要

Using the radiomimetic drug, bleomycin, we have determined the mutagenic potential of DNA strand breaks in the shuttle vector pZ189 in human fibroblasts, The bleomycin treatment conditions used produce strand breaks with 3'-phosphoglycolate termini as >95% of the detectable dose-dependent lesions, Breaks with this end group represent 50% of the strand break damage produced by ionizing radiation. We report that such strand breaks are mutagenic lesions. The type of mutation produced is largely determined by the type of strand break on the plasmid (i.e. single versus double), Mutagenesis studies with purified DNA forms showed that nicked plasmids (i.e. those containing single-strand breaks) predominantly produce base substitutions, the majority of which are multiples, which presumably originate from error prone polymerase activity at strand break sites, In contrast, repair of linear plasmids (i.e. those containing double-strand breaks) mainly results in deletions at short direct repeat sequences, indicating the involvement of illegitimate recombination. The data characterize the nature of mutations produced by single- and double-strand breaks in human cells, and suggests that deletions at direct repeats may be a 'signature' mutation for the processing of DNA double-strand breaks.