Functional characterization of cis- and trans- regulatory elements involved in expression of phospholipid hydroperoxide glutathione peroxidase

摘要

Phospholipid hydroperoxide glutathione peroxidase (phGPx) is a member of the seleno glutathione peroxidase family that is comprised of five seleno-proteins capable of reducing hydroperoxy lipids to the corresponding alcohols. The enzyme has been implicated in antioxidative defense, but its high expression level in testicular tissue suggests a more specific function during sperm maturation. The phGPx is encoded for by a joint sperm nucleus/phGPx gene (sn/phGPx) and can be expressed as a mitochondrial or cytosolic isoform. Although sn/phGPx genes have been cloned from various mammalian species expression regulation of the enzyme has not been studied in detail. We investigated the 5'-flanking region of the murine sn/phGPx gene and observed basic promoter activity in a 200 bp region localized immediately upstream of the translational initiation site of the cytosolic isoform (3'-ATG). DNase protection assays indicated the presence of five distinct protein-binding regions and electrophoretic mobility shift assays and supershift experiments revealed binding of stimulating protein 1 (SP1), nuclear factor Y(NF-Y) and members of the SMAD family. Site-directed mutagenesis of the consensus binding sequences abolished in vitro transcription factor binding. Expression of reporter genes was most effectively impaired when SP1/SP3 and NF-Y binding site-deficient constructs were tested. Chromatin immunoprecipitation suggested the in vivo relevance of these transcription factors. Our data indicate that the basic phGPx promoter constitutes a 200 bp oligonucleotide, which is localized immediately upstream of the 3'-ATG and involves functional SP1/SP3, NF-Y and SMAD binding sites. The corresponding trans-regulatory proteins may contribute to differential expression regulation of the mitochondrial and cytosolic phGPx isoforms.