A fluorescence study of (4-{(lE,3E)-4-[4-(dimethylainino)phenyl]-buta-l,3-dienyl}phenyl)methanol and its bioconjugates with bovine and human serum albumins

摘要

Electronic absorption and fluorescence properties of 4-{(lE,3E)-4-[4-(dimethylamino)phenyl]buta-l,3-dienyl}phenyl)methanol in different media,including organic solvents of varying polarity at 298 K,an ethanol-methanol matrix at 77 K and protein environments,have been examined.The diene shows solvatochromic fluorescence,which is correlated with empirical solvent polarity parameters.The red-shifted fluorescence emission of the diene is attributed to a polar,conformationally relaxed intramolecular charge transfer excited state.Further,the diene has been covalently attached to bovine serum albumin and human serum albumin and the fluorescence probe properties of the protein-diene conjugates have been examined in phosphate buffer.