Cryopreservation of yellow-poplar and sweetgum embryogenic cultures

摘要

Cryopreservation has become an essential tool for operational application of forest tree embryogenic cultures, due to the long evaluation periods needed for trees regenerated from these cultures. Five yellow-poplar (Liriodendron tulipifera) and seven sweetgum (Liquidambar spp.) embryogenic culture lines were stored in liquid nitrogen for 48 hours, after which they were thawed and tested for regrowth and ability to produce somatic seedlings. Combinations of two sorbitol pretreatments and three dimethylsulfoxide (DMSO) cryoprotectant levels were evaluated for their impact on recovery following cryogenic storage. The best results were obtained with 0.4 M sorbitol and 5% DMSO, which provided 100% recovery. Somatic seedlings were regenerated from all culture lines and treatments, except for a transgenic sweetgum line.