Mathematical Modeling of the Effects of N-Terminal Fragmentsof Brain Immunophilin that Affect Phospholipid MetabolismDisorders in Mycotoxin Zearalenone Intoxication

摘要

Experimental studies have been performed with the brain and spleen lymphocyte membranes (LM) of white rats intoxicated with mycotoxin zearalenone (MTZ). The results demonstrated strong activation of free radical oxidation (FRO) processes in lipids of the LM. The quantitative changes in malonic dialdehyde (MDA) were determined during the first five hours in the brain and spleen LM of the intoxicated rats. MTZ is a powerful activator of these pathogenic processes.The goal of this research was to study the antioxi-dant effects of different compounds (vitamin E, sodium thiosulfate, and a synthetic fragment of brain immunophilin (IPh) (1-15)) in the normalization of the lipid peroxidation processes. We determined the amino acid sequence of the N-terminal region, synthesized this fragment, and the studied its effects on the brain and spleen of white rats intoxicated with MTZ.It was essential to examine the effects of factors such as the IPh concentration and exposure interval on the processes of lipid FRO in the intoxicated body. To this end, we analyzed the effects of the interaction between those two factors. Based on the data obtained, we developed a mathematical model based on statistical methods and expressed the observed biological phenomena as mathematical equations, which allowed us to predict the quantity of MDA after application of N-terminal IPh (1-15).