Simultaneous ultrasound-assisted water extraction and beta-cyclodextrin encapsulation of polyphenols from Mangifera indica stem bark in counteracting TNFa-induced endothelial dysfunction

摘要

This study proposes an alternative technique to prevent heat degradation induced by classic procedures of bioactive compound extraction, comparing classical maceration/decoction in hot water of polyphenols from Mango (Mangifera indica L.) (MI) with ultrasound-assisted extraction (UAE) in a water solution of -cyclodextrin (-CD) at room temperature and testing their biological activity on TNF-induced endothelial dysfunction. Both extracts counteracted TNF effects on EAhy926 cells, down-modulating interleukin-6, interleukin-8, cyclooxygenase-2 and intracellular adhesion molecule-1, while increasing endothelial nitric oxide synthase levels. -CD extract showed higher efficacy in improving endothelial function. These effects were abolished after pre-treatment with the oestrogen receptor inhibitor ICI1182,780. Moreover, the -CD extract induced Akt activation and completely abolished the TNF-induced p38MAPK phosphorylation. UAE and -CD encapsulation provide an efficient extraction protocol that increases polyphenol bioavailability. Polyphenols from MI play a protective role on endothelial cells and may be further considered as oestrogen-like molecules with vascular protective properties.