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首页> 外文期刊>Nephrology, dialysis, transplantation: official publication of the European Dialysis and Transplant Association - European Renal Association >Regulation of the transcription of parathyroid-hormone/parathyroid-hormone-related peptide receptor mRNA by dexamethasone in ROS 17/2.8 osteosarcoma cells.
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Regulation of the transcription of parathyroid-hormone/parathyroid-hormone-related peptide receptor mRNA by dexamethasone in ROS 17/2.8 osteosarcoma cells.

机译:地塞米松在ROS 17 / 2.8骨肉瘤细胞中对甲状旁腺激素/甲状旁腺激素相关肽受体mRNA转录的调节。

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摘要

Previous studies have shown that dexamethasone enhanced the expression of parathyroid-hormone/parathyroid-hormone-related peptide (PTH/ PTHrP) receptor mRNA in ROS 17/2.8 osteosarcoma cells. The aim of this study was to determine whether the induction of PTH/PTHrP receptor expression in such osteoblast-like cells is regulated at the gene level. Dexamethasone increased the steady-state levels of PTH/PTHrP receptor mRNA twofold at 6 h, and nearly threefold at 24 h. The half-life of the PTH/PTHrP receptor mRNA, in the presence of actinomycin D, was 6 h both in untreated and in dexamethasone-treated cells. When measured by nuclear run-on assay, the rate of PTH/PTHrP receptor gene transcription was increased twofold at 24 h. PTH/PTHrP receptor mRNA expression was blocked completely after 24 h of treatment with cycloheximide. The binding of PTH/PTHrP to their receptor required the synthesis of new protein and was shown to be specifically dependent on the interaction of dexamethasone with the glucocorticoid receptor. These data indicate that the enhancing effect of dexamethasone on PTH/PTHrP receptor expression is rapid, requires de novo protein synthesis, and increases the transcription rate of the PTH/PTHrP receptor gene.
机译:先前的研究表明,地塞米松可增强ROS 17 / 2.8骨肉瘤细胞中甲状旁腺激素/甲状旁腺激素相关肽(PTH / PTHrP)受体mRNA的表达。这项研究的目的是确定在这种成骨样细胞中PTH / PTHrP受体表达的诱导是否在基因水平上受到调节。地塞米松使PTH / PTHrP受体mRNA的稳态水平在6小时增加了两倍,在24小时增加了近三倍。在放线菌素D存在下,PTH / PTHrP受体mRNA的半衰期在未经处理和地塞米松处理的细胞中均为6小时。当通过核运行测定法测量时,PTH / PTHrP受体基因转录的速率在24小时增加了两倍。用环己酰亚胺治疗24小时后,PTH / PTHrP受体mRNA的表达被完全阻断。 PTH / PTHrP与它们的受体的结合需要合成新的蛋白质,并且被证明特别依赖于地塞米松与糖皮质激素受体的相互作用。这些数据表明,地塞米松对PTH / PTHrP受体表达的增强作用是快速的,需要从头合成蛋白质,并增加了PTH / PTHrP受体基因的转录速率。

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