Ni-NTA-coated nanowire materials for protein enrichment and the application in a medical device used for blood glucose degradation

摘要

A general and reliable method has been developed to functionalize either the iron oxide or the silicon nanowires (NWs) with nickel-nitriloacetic acid (Ni-NTA) complex, which was manufactured to manipulate His-tagged proteins and enzymes. The Ni-NTA-functionalized sea-urchin-shaped α-Fe _2O_3 NWs exhibit the superior protein purification efficiency and excellent stability in the form of dry powder. Application of this new nanotechnology in biomedical research field has been explored. A glucose degradation bio-matrix was made via the Ni-NTA-modified silicon NW-chips, which were conjugated with an enzyme essential to glycolysis. The glucose level in a simulated blood solution was found to be reduced from 14.4 mM to 9 mM after incubating the hexokinase I-functionalized silicon NW-chips for 12 h. These results suggest a possible way to build up a medical device using enzymes functionalized NW-chips for the removal of excess blood glucose.