Phylogenetic (16S rDNA) analysis of a halophilic actinobacteria, isolated from Pitchavaram mangroves of India, claims the genus Actinopolyspora

摘要

Sediments were collected from 9 different saline habitats of the Pitchavaram mangrove ecosystem, India, to isolate halophilic actinobacteria, using three different media. The halophilic actinobacteria were observed only at station 9 (Suaeda region with the salinity of 65%o) and only in the medium 1. Out of 11 strains isolated, one strain nl was selected for detailed taxonomical investigations. The strain (nl) required higher concentration of sodium chloride (17 to 22 % (w/v) for optimal growth and thus ascertaining it's halophilic nature. The 16S rDNA analysis of the. strain provided with a strong evidence to prove that the strain nl is phylogenetically akin to the genus Actinopolyspora,. Micromorphological characters of the strain nl also resembledthe genus Actinopolyspora and thus warranting it's inclusion in the genus Actinopolyspora. The Gene Bank nucleotide sequence accession number of the strain nl is AY 015427.