Genetic Relationships of Korean Astringent Persimmon VarietiesUsing AFLP Analysis

摘要

Astringent type persimmon varieties have been cultivated from ancient times in Korea. In the long cultivation of the persimmon, various local varieties have been developed and cultivated throughout from the central to the Southern area. Therefore, theaim of this study was to reveal genetic relationships of local 22 persimmon varieties, including one Chinese cultivar 'Damopan' and four Japanese cultivar 'Koshuhyakume', 'Hirataneanshi', 'Saijo', and 'Yokono', using AFLP technique. Total bulk DNA of the persimmon cultivars were amplified with primer combinations based on the sequence of EcoRI and Msel adapters. The eight primer combinations amplified a total of 357 polymorphic fragments. According to AFLP calculated genetic similarities, 'Godongsi' and 'Hakdongsi' were closely related each other, hi addition, 'Durigam' and 'Yokono' showed close genetic similarity, which were collected from Hamyang in Korea and Japan, respectively. However, Chinese persimmon 'Dampen' and 'Wolhasi' were poorly relatedwith the other collected varieties.