Immunoblotting Assay for Glyphosate-tolerant Genetically Modified Soybean in Soybean Products

摘要

Genetically modified (GM) soybean Roundup Ready carries Agrobacterium sp. CP4 gene, which expresses 5-enolpyruvylshikimate-3-phosphate synthase (CP4EPSPS) CP4EPSPS in GM soybeans and soybean curds was screened using CP4EPSPS-specific polyclonal and monoclonal antibodies (pab and mab, respectively) by immunoblotting. Isolated recombtnant CP4EPSPS was detected at detection limits of 0.006 and 0.0006 μg, whereas those of CP4EPSPS expressed in GM soybean were 0.001 and 0.0001 μg, using mab and pab, respectively, From nine screened soybean curds, two had positive results with pab. Immunoblotting method with pab and mab developed in this study could be applied to screen glyphosate-tolerant GM soybeans in soybean products.