A study on dietary supplement to reduce obesity by the mechanism of decreasing lipid and carbohydrate absorption

摘要

Pancreatic lipasc and a-amylase inhibitory activities in purified extracts of pumpkin and job's tear were studied. Pancreatic lipase inhibitory activity was determined by measuring the rate of releasing oleic aeid from triolein, and a-amylase inhibitory activity was determined by iodometric method. The extracts of pumpkin and job's tear were purified using silica gel and C-18 gel column chromatographies. Treatment of pumpkin extract (120 mu g/mL) in 3T3-L1 preadipoeyte decreased differentiation about 95 percent and blocked accumulation of lipid. Body weights of rats fed high-fat diet containing dietary supplement decreased about 13 percent as compared with those fed only high-fat diet. These results revealed dietary supplement is a good obesity-reducing material for decreasing lipid and carbohydrate absorptions.