On-line analysis of Phellinus linteus WI-001 fermentation parameters

摘要

Fermentation parameters were estimated by use of a vent gas analyzer coupled to a computer data acquisition system in cultivation of Phellinus linteus WI-001, a producer of polysaccharides known to have potent anticancer activities. Oxygen uptake rate (OUR), a critical indicator of the cells' activities, was calculated by applying oxygen mass balance. In addition, by dividing the oxygen uptake rate by the total oxygen consumed, on-line estimation of the cells specific growth rate was successfully done. It was also possible to estimate cell concentration directly by use of oxygen-cell yield (Y_(x/o)) which was obtained based on a correlation between cell growth and total oxygen consumed.