Characterization of extracellular protease of Bacillus sp. WRD-1 isolated from soil

摘要

Alkaline bacterium producing a high protease activity at low temperature was isolated by using enrichment culture from soil samples and identified as Bacillus sp. WRD-1. Cell growth was maximal at 10 hours and the optimal initial pH and culture time of culture condition for enzyme production was pH 7 and 10 hours, respectively. Temperature range of high enzyme activity were 10 - 40 deg C. The optimal pH and temperature for the enzyme activity were pH 9 and 30 deg C.