Production of compactin from Penicillium sp. Y-8515

摘要

A strain producing high levels of compactin was isolated from soil and identified as Penicillium sp. Y-8515 based on the morphological characteristics and ribosomal RNA sequence analysis. Optimization of several different carbon and nitrogen sources for the effective production of compactin was performed resulting in the medium compositions containing 5 percent(w/v) glucose, 1.0 percent soybean meal, 0.5 percent yeast extract, 0.5 percent (NH_4)SO_4, 0.25 percent NaH_2PO_4, 0.25 percent CaCO_3. The fixed concentration of glucose (5 percent, w/v) and relatively lower concentrations (less than 2.5 percent, w/v) of soybean meal stimulated the transformation of the growth morphology from filamentous to pellet form. Comparing to that by filamentous form, the production of compactin by pellet form increased up to 1.5 folds. In a fed-batch fermeritation, continuous feeding of the mixture of glucose and nitrogen source at the ratio of 10:1 showed 3.5-fold more production yield of compactin comparing to the batch mode.