IMMOBILIZATION OF PENICILLIN ACYLASE OF FILAMENTOUS FUNGI ON Opuntia imbricata

摘要

The immobilization of the extracellular penicillin G acylases (PGA) from filamentous fungi (Aspergillus fumigatus and Mucor griceocianus) on the Opuntia imbricata is reported in this paper.O.imbricata is a cactus,also known as coyonoxtle,and is considered as invasive plant in the North of Mexico.The immobilization of the enzyme was carried out after activation of coyonoxtle with different periodate concentrations in order to provide chemical attachment of the enzyme.The results obtained in the present study showed that O.imbricata offered great possibilities for enzyme immobilization.In the case of PGA from filamentous fungi,high levels of protein immobilization and relative activity were demonstrated.Likewise an improvement in the operational and storage stability of the enzyme was observed.However,the activation of support by means of periodate did not demonstrate any appreciable effect on the properties of immobilized enzyme.This probably could be due to the reason that the immobilization of penicillin acylase on O.imbricata was more of adsorption equilibrium than by the chemical attachment.