Screening of the Culture Media with Different Concentrations of Nutrients for Cultivation of the Microalgae Associated with the Invertebrates of the White Sea

摘要

The growth and biomass accumulation of three microalgal strains of Desmodesmus (Scenedesma-ceae, Chlorophyceae), lPm66B, 2C166E, and 3Dp86E-l, isolated from the White Sea benthic invertebrates were studied under conditions of batch culture indifferentstandard media (BG-11, Prat, Goldberg, Gromov, Tamiya, artificial seawater) and modified media. The culture condition, biomass accumulation, and uptake of nitrate and phosphate were recorded. A significant alkalization of the culture medium up to pH 10has been observed during a vigorous growth of the microalgae. The most significant biomass accumulation has been recorded in BG-11 (in complete or modified medium with addition of artificial seawater), Tamiya, and Prat media. Addition of seawater did notaffect the growth of Desmodesmus sp. in the nitrate-containing media, although that maintained growth of the microalgae in the nitrogen-lacking media without cell aggregation. The BG-11 medium appears suitable for isolation and cultivation of both symbiotic and free-living microalgae by all the tested features. The Prat medium is the best for maintaining the microalgal strains in living collection.