BAP37 and prohibitinare specifically recognized by an SV40 T antigen antibody

摘要

We have identified two cellular proteins that are specifically immunoprecipitated by an anti-SV40 T antigen monoclonal antibody.This antibody,PAb419,recognizes an epitope contained within a region of T antigen which we have recently demonstrated is re-quired for the initiation of immortalization by SV40 Tantigen,but is not essential for maintenance of the immortal state.The two proteins were identified as BAP37 and Prohibitin.Recent results suggest Prohibitin may enhance the transcriptional inactivation of E2F by the retinoblastoma family of pocket proteins (pRb,p107,p130).BAP37 and Prohibitin are specifically recognized by PAb419 and PAb210,another anti-SV40 T antigen monoclonal antibody,which has an overlapping epitope,but not by other anti-SV40 T an-tigen monoclonal antibodies,demonstrating the specificity of the interaction.