Expression and location of taurine transporters and channels in the epididymis of infertile c-ros receptor tyrosine kinase-deficient and fertile heterozygous mice.

摘要

Defective sperm volume regulation causes infertility in c-ros knockout (KO) mice lacking the initial segment of the epididymis (IS). As taurine is in high concentration in the male tract and acts as an organic osmolyte in somatic cells, the taurine transporter (TauT), taurine content and taurine channel phospholemman (PLM) were examined in the epididymides of these and fertile heterozygous (+/-) mice. TauT and PLM genes were demonstrated by RT-PCR in the caput of +/- and c-ros knockout males. Northern blots revealed one transcript of TauT (8 kb) in all regions with different expression between segments (cauda > corpus > IS > caput) and no differences in expression between genotypes. Western blotting revealed three translation products of TauT in all epididymal regions (107, 69, 49 kDa) with higher expression of the 69 kDa and 49 isoforms in the -/- than +/- caput. Immunohistochemical staining revealed staining of principal cells and stronger staining of apical and clear cells in all epididymal regions.The expression of the PLM transcript (0.75 kb: cauda = corpus > caput > IS) was upregulated in the proximal caput and cauda of KO mice. Tissue taurine was higher in the cauda >corpus>IS congruent with caput in fertile males and significantly higher in the proximal caput of the KO male. By contrast, taurine concentrations in cauda epididymidal fluid and content per 10(6) sperm did not differ between genotypes. TauT and PLM may be involved in taurine regulation in the normal epididymis and the proximal accumulation of taurine in the infertile males. Mol. Reprod. Dev. 64: 144-151, 2003.