Isolation and characterization of eight microsatellite loci in the icefish Chaenocephalus aceratus (Perciformes, Notothenioidei, Channichthyidae)

摘要

We characterized eight polymorphic microsatellites in the Scotia icefish Chaenocephalus aceratus (Perciformes, Notothenioidei, Channichthyidae) that is endemic of Southern Ocean waters surrounding the tip of the Antarctic Peninsula. Microsatellites were isolated from a partial genomic library enriched for an AC motif. The number of alleles ranged from two to 19 with a mean observed hererozygosity of 0.71. Loci were in Hardy-Weinberg equilibrium and no evidence for linkage disequilibrium was found. These molecular markers will be useful to investigate Scotia icefish genetic structure, possibly providing insights on its effective population size and demographic history.