Polymorphic microsatellite loci isolated from the marine sponge Scopalina lophyropoda (Demospongiae : Halichondrida)

摘要

A total of seven microsatellites out of 88 isolated from a genomic library enriched for (CA),, and (GA)(n) repeats were characterized in the Mediterranean marine sponge Scopalina lophyropoda. The microsatellite motifs were large (34.81 +/- 13.9 bp) and imperfect. The seven microsatellite loci were screened in 30 individuals collected from Blanes, northwestern Mediterranean. All of them were polymorphic (allele numbers and observed heterozygosities ranged from 3 to 6 and from 0.16 to 0.76, respectively). No significant linkage disequilibrium between pairs of loci and no departure from Hardy-Weinberg equilibrium were found. These markers are therefore promising for studies of the population structure of the species.