Regulatory B cells from hilar lymph nodes of tolerant mice in a murine model of allergic airway disease are CD5 ~+, express TGF-β, and co-localize with CD4 ~+ Foxp3 ~+ T cells

摘要

In a biphasic, ovalbumin (OVA)-induced murine asthma model where allergic airway disease is followed by resolution and the development of local inhalational tolerance (LIT), transforming growth factor (TGF)-β-expressing CD5 ~+ B cells were selectively expanded locally in hilar lymph nodes (HLN) of LIT mice. LIT HLN CD5 ~+ B cells, but not LIT HLN CD5 ~+-B cells, induced expression of Foxp3 ~+ in CD4 ~+ CD25-T cells in vitro. These CD5 ~+ regulatory B cells (Breg) and CD4 ~+ Foxp3 ~+ T cells demonstrated similar increases in expression of chemokine receptors (CXCR4 and CXCR5) and co-localized in HLN B cell zones of LIT mice. The adoptive transfer of LIT HLN CD5 ~+ B cells, but not LIT HLN CD5 ~+-B cells, increased the number of CD4 ~+ Foxp3 ~+ T cells in the lung and inhibited airway eosinophilia in this OVA model. Thus, Breg in HLNs of LIT mice reside in a CD5 ~+ TGF-β-producing subpopulation and co-localize with CD4 ~+ Foxp3 ~+ T cells.