Proteomic Mapping of Living Cells with An Evolved Peroxidase Reporter, and Application to Mitochondrial and Endoplasmic Reticulum Proteomes

摘要

A challenge to obtaining high quality organellar proteomes is the difficulty of purifying organelles to homogeneity without loss of crucial components. Furthermore, many subcellular regions of great importance cannot be purified at all, and hence their proteomes are not known. We present a live cell proximity-dependent enzymatic biotinylation strategy that bypasses the need for organellar purification and produces highly specific proteomic maps of multiple subcellular regions.