Genetic Purity of Interspecific Hybrids of Solanum melongena and S. macrocarpon Assessed Using SSR Markers

摘要

Traditionally hybrid purity of Fi hybrids is assessed through grow-out tests, which are time consuming and labor intensive. There is need for a fast and reliable alternative method for hybrid purity testing. The objective of this work was to assess purity of interspecific hybrids of Solanum melongena L. ('IIHR3', Arka Keshav OAK'), '2BMG') and Solanum macrocarpon L. CSM') using simple sequence repeats (SSR). Genomic DNA from parents and Fj hybrids were subjected to SSR analysis to detect parental polymorphism. Among 119 SSRs screened, 5 SSRs were codominant. There were five unique microsatellite markers, two for TIHR3' x'SM', emf 01C03 and emh 02E08; one for 'AK' x'SM', emi 02 F16; and two for '2BMG' x'SM', emb 01E 03 and emg 11103, which were useful to detect purity of three interspecific eggplant hybrids. This is the first report demonstrating the utility of SSR markers for assessment of genetic purity of interspecific hybrids in eggplant.