Piezo-resistive method for tuberculosis detection using microcantilever biosensor

摘要

Microcantilever device is utilized in several biosensors for detection of bio-molecule of interest. Biosensors task is to identify presence of targeted molecule and supply result into a measurable signal. Comparing with conventional biological equipments, micro scale biosensors are very fast, reliable and price effective. Microelectro-mechanical systems (MEMS)anoelectromechanical systems are used for designing of such biosensors, which might be utilized in several biological applications. Sensing mechanism of biosensor is varied with the application. This paper is focused on detection of bio-molecule using microcantilever beam. Biosensor has shown here uses piezo-resistive method for detection of targeted bio-molecule. This Bio-MEMS device is designed and simulated using coventorware software. This biosensor wants to identify a presence of T.B. in a very suspected patient. For detection of primary stage of tuberculosis (ESAT-6), specific antibodies need to immobilize on top of microcantilever. Once patient sample (consist of ESAT-6) is placed on functionalized cantilever surface, biochemical reaction happen between tuberculosis antigens ESAT-6 and antibodies. Adsorption of antigens will increase mass working on cantilever and results in bending of microcantilever. This deflection shows the presence of tuberculosis within the patient sample.