Brilliant cresyl blue as a new red region fluorescent probe for determination of nucleic acids

摘要

A fluorescence quenching method was developed for determination of icroamounts of nucleic acids by using brilliant cresyl blue (BCB) as a new red region fluorescent probe.In aqueous hexylmethylene tetramine solution,BCB showed maximum excitation and emission wavelengths at 626 and 670 nm,respectively,andthe fluorescence of BCB could be greatly quenched by DNA (or RNA).Under optimal conditions,the calibration graphs are linear over the range of 0.02-0.80 #mu#g/ml for SM DNA and 0.25-1.5 #mu#g/ml for yeast RNA.The corresponding detection limits are 7 ng/ml for SM DNA and 25 ng/ml for yeast RNA,respectively.SM DNA can be determinated in the presence of 40% (w/w) RNA,and the relative stadard deviation of six measureemnts is 2.5% for 500 ng/ml SM DNA.The result of he determination of golden staphylococcus DNA by this method was satisfactory.