Indirect method for quantitative determination of bovine serum albumin and transferin by anodic stripping voltammetry with a rotating glassy carbon electrode

摘要

The present study was carried out in order to investigate whether the interaction of multifunctional transport proteins, bovine serum albumin (BSA) and transferin (TF), with some metal (Me) ions, such as Hg(ll) and cum), in buffered solution (pH 8.0), may serve as a base for indirect quantitative determination of both proteins. For this purpose anodic stripping voltammetry with a rotating glassy carbon electrode was applied. A sufficient sensitivity on thc presence of protein in solution containing Me-ions was obtained only by using a mercury dissolution signal (peak) obscrved at 0.1 V vs. SCE. Quantities of BSA less than 5 X 107 M were determined with a S.D+-5% (n = 10) for a concentration of 3 x10~(-7) M, and for TF the concentration less than 1.5 X 10~(-7) M could be determined with S.D +- 5% (n = 10) fora concentration of.1 X 10~(-7) M.