Evaluation of diphenyleneiodonium influence on cardiac morphology and selected redox equilibrium markers in rats treated with doxorubicin

摘要

The aim of the study was to evaluate the effect of diphenyleneiodonium sulfate (DPI) on cardiac morphology and oxidative stress secondary to doxorubicin administration. Rats were intraperitoneally injected with doxorubicin (1.5 mg/kg) once a week for ten weeks (DOX group). In each case, DPI was administered subcutaneously at two single doses (0.25 or 1 mg/kg), 24 h and 4 h before and 24 h after doxorubicin injection (DPI+DOX groups). Necropsy was performed three weeks after the completion of doxorubicin treatment. DPI significantly reduced the cardiac NADPH level and, at the higher dose, normalized cardiac lipid peroxidation altered by doxorubicin. There were no significant changes in the levels of cardiac NADH, glutathione, nor differences in plasma cardiac troponin I, fatty acid binding protein, and B-type natriuretic peptide, between the DOX and DPI+DOX groups. No cardiomyocyte necrosis was observed in biochemical and morphological examinations. However, DPI highly augments other cardiac morphological changes caused by doxorubicin.