Effect of amphotericin B on the biochemical markers in the haemolymph of honey bees

摘要

We examined the effect of amphotericin B (AmB) on the following enzymatic markers: aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), and on non-enzymatic markers: glucose, triglycerides, and proteins in the haemolymph of a model organism, Apis mellifera. AmB is an antifungal antibiotic. Despite its toxicity, it is used to treat disease conditions. The haemolymph of honey bees is considered as an analogue of human blood, and changes in marker values indicate pathological states, both in humans and honey bees. Three groups of caged bees were fed sugar syrup (the control group). The syrup was supplemented with AmB at concentrations of 0.25 mg/ml (AmB-25) and 0.50 mg/ml (AmB-50). The authors observed that the biochemical markers were age-related in the control group. Decreased values of the enzymatic markers in the AmB-treated groups confirm that AmB has a negative effect on the organism. The higher the dose of the antibiotic, the greater the increase in the concentration of the non-enzymatic markers. Our research shows that honey bees are an important model for studying the effects of AmB.