Simple and Rapid Regeneration of Plumbago rosea using BAP for Direct and Indirect Plant Regeneration from Leaf and Nodal Explants

摘要

A rapid and highly effective method for plant micropropagation from leaf bits and vegetative shoot buds was established for the medicinal plant Plumbago rosea Linn. The number of multiple shoots per explant ranged from 8-10 when cultured on Murashigeand Skoog (MS) basal medium supplemented with 0.5 mgl~(-1) indole-3-butyric acid (IBA) and 6 mgl~(-1) 6-benzylamino purine (BAP). Nodal explants initially produced basal callus followed by 10 ± 0.71 multiple shoots. Recently matured leaves i.e., third leaves from the top, when cultured on MS with 0.25 mgl~(-1) BAP, 0.25 mg~(-1) kinetin and 0.5 mgl~(-1) ascorbic acid and maintained in the dark, produced dark green calli which in later stages produced shoot buds in a 16-h photoperiod regime. About 12 ±1.22 multiple shoots also developed directly within 15 days from the leaf margin as well as from the wounds when maintained at a 16-h photoperiod regime. More than 85% of the matured leaf explants regenerated shoot buds, while very young or fully mamre leaves did not respond. Excised shootlets cultured on half-sfrength MS with 0.2 mgl~(-1) IBA rooted within 45 days. Successful transfer of the rooted shots to pots was accomplished with 100% survival.