Calibration standards and phantoms for fluorescence optical measurements

摘要

We report on the development and production of tissue-like optical phantoms for detection and characterization of fluorescent objects close to the tissue surface. The phantoms mimic bulk optical properties such as light scattering and absorption of female breast tissue and human skin. Fluorescence sources are embedded for a given well-defined localization and volume to simulate pathologic lesions that are marked by specific molecular substances labeled with a fluorescent dye. The phantoms were made of epoxy resin with added titanium dioxide and black epoxy color paste for adjustment of the tissue-like scattering and absorbing properties. Indocyanine green was used as a fluorescent dye. The phantoms were evaluated with an experimental set-up for a new method for in vivo laser fluorescence tomography in the frequency domain.