Efficient Gene Carriers Composed of 2-Hydroxypropyl-β-Cyclodextrin, Ethanolamine-Functionalized Poly(glycidyl methacrylate), and Poly((2-dimethyl amino) ethyl methacrylate) by Combination of ATRP and Click Chemistry

摘要

In this work, a simple one-step method is first employed to produce the bromoisobutyrylterminated 2-hydroxypropyl-b-cyclodextrin (HPCD-Br). The pendant epoxy groups of poly- (glycidyl methacrylate) block prepared via ATRP from HPCD-Br can be reacted with ethanolamine to produce HPCD-PGEA which exhibits much lower cytotoxicity and better gene transfection yield than polyethylenimine (25 kDa) in COS7 and HepG2 cell lines. Moreover, poly((2-dimethyl amino)ethyl methacrylate) blocks can be incorporated into low-molecularweight HPCD-PGEA via "click" reaction to further enhance the gene transfection efficiency in HepG2 cell lines.