Vitamin E requirements of juvenile grass shrimp, Penaeus monodon, and effects on non-specific immune responses

摘要

A feeding trial was Conducted to determine the dietary vitamin E (DL-alpha-tocopheryl acetate, DL-alpha-TOA) requirement and its effect on the non-specific immune responses of juvenile grass shrimp, Penaeus monodon. Purified diets with eight levels (0. 25. 50. 75 100 150 200 400 mg vitamin E kg diet(-1)) of supplemental DL-alpha-TOA were fed to P. monodon (mean initial weight 0.29 +/- 0.01 g) for eight weeks. Each diet was fed to three replicate groups of shrimp. Weight gains and total haemocyte count (THC) were higher (P < 0.05) in shrimp fed diets Supplemented with 75 and 100 mg vitamin E kg diet(-1) than in shrimp fed diets supplemented with < 50 mg vitamin E kg diet(-1). Superoxide dismutase (SOD) activity was higher in shrimp fed diets Supplemented with 50-200 mg vitamin E kg diet(-1) than in shrimp fed diets supplemented with < 25 and 400 mg vitamin E kg diet(-1). Vitamin E concentrations in the hepatopancreas and muscle of the shrimp were increased Lis the dietary vitamin E supplementation level was increased LIP to 200 mg vitamin E kg diet(-1). Thiobarbituric acid (TBA) values in the hepatopancreas and Muscle were generally decreased as the dietary vitamin E supplementation level increased. Analysis by polynomial regression of weight gain, THC and hepatopancreatic vitamin E concentration of the shrimp indicated that a level of 85-89 mg vitamin E kg diet(-1) is required for maximal growth and non-specific immune responses of P. monodon and that 179 mg vitamin E kg diet(-1) is required to maximise tissue vitamin E concentration