Immunotoxic and cytotoxic effects of atrazine, permethrin and piperonyl butoxide to rainbow trout following in[NON-BREAKING SPACE]vitro exposure.

摘要

For many current use pesticides, limited information exists on their cytotoxicity and immunotoxicity in non-target organisms such as fish. We examined the effects of atrazine, permethrin and piperonyl butoxide (PBO) exposure, in[NON-BREAKING SPACE]vitro, on rainbow trout (Oncorhynchus mykiss) lymphocyte viability and proliferation. Purified rainbow trout peripheral blood leukocytes (PBLs) were exposed in[NON-BREAKING SPACE]vitro to the test chemicals (0, 0.01, 0.1, 1 and 10[NON-BREAKING SPACE]muM) for 96[NON-BREAKING SPACE]h, with and without the mitogen lipopolysaccharide. All three chemicals caused a decrease in both lymphocyte viability and proliferation at 10[NON-BREAKING SPACE]muM, while atrazine also suppressed proliferation of PBLs at 1[NON-BREAKING SPACE]muM. The in[NON-BREAKING SPACE]vitro toxicity of these chemicals to this salmonid underscores the need for further investigation using in[NON-BREAKING SPACE]vivo studies and host resistance models