Effects of Cadmium Salts on the Reproductive Potential of Male Rainbow Trout as Determined by in Vivo and in Vitro Techniques.

摘要

A new method for dispersing strongly adhesive cells which uses trypsin and EDTA and ultrasonic sound at non-destructive levels,has been applied to cultures of rainbow trout gonad (RTG-2) cells and has yielded monodisperse cells. Cadmium chloride was added to Liebovitz L-15medium to give concentrations of 0, 1, 5and 10mg CdCl2/1to determine the effect of Cd on the growth rate and morphology of rainbow trout gonad cells. One mg CdCl2/1had very little effect on the cells with growth rates and morphology equivalent to control cells lacking CdCl2. After 6days contact with 5mg CdCl2/1, a depression in growth rate below control levels was observed as well as morphologic change in cells characterized by decreased substrate adhesion and the formation of thin granular cellular projections. At 10mg CdCl2/1 a rapid loss of cells occurred with a loss of cellular integrity.