Effect of methylene bisthiocyanate on propugales and established mycelium of two sapstain fungi

摘要

The effect of methylene bisthiocyanate (MET) on Sphaeropsis sapinea and Ophiostoma floccosum was investigated using wood wafer assays and chemical analysis. Results obtained demonstrated that O, floccosum was significantly more tolerant to MET than S.sapinea (p <= 0.001). O. floccosum tolerated MET levels at least four times greater than S. sapinea. Established hyphae of both test fungi had greater tolerance against MET as compared to their respective propagules (spores/hyphal fragments). Volatile components released from MBT-treated wood inhibited fungal growth on untreated wafers. Headspace analysis using gas chromatographic-mass spectroscopy confirmed the volatile components released from MET treated wood. However, the combined effect of diffusion and vapor action of MET was more pronounced in controlling fungal growth.