Simultaneous determination of trimethoprim and diaveridine in tissues of chicken, porcine, and fish by hydrophilic interaction liquid chromatography-tandem mass spectrometry.

摘要

A sensitive liquid chromatography-tandem mass spectrometry method for simultaneous determination of trimethoprim and diaveridine in edible tissue samples (chicken and porcine muscle; liver, kidney, and fat tissues as well as fish muscle) was developed and validated in this study. Trimethoprim, diaveridine, and the internal standard trimethoprim-D₃ were extracted from samples using acetonitrile. The Oasis MCX Solid-Phase Extraction Cartridge was selected for cleaning up the extracts. Chromatographic separation was performed on a hydrophilic interaction liquid chromatography column with gradient elution. The analytes were determined using triple-quadrupole mass spectrometry in positive electrospray ionization and multiple reaction monitoring mode. The relative recoveries from spiked samples ranged from 82.5 to 117.2 %, with the relative standard deviations generally being below 15.4 %. Limits of detection and quantification for analytes were within 0.3-1 and 1-2 mug kg-1, respectively. The proposed method was successfully applied to detect trimethoprim and diaveridine in real samples. copyright Springer Science+Business Media New York 2013.