Involvement of macrophage colony-stimulating factor (M-CSF) in the function of follicular granulosa cells.

摘要

OBJECTIVE: To evaluate the role of macrophage colony-stimulating factor (M-CSF) in steroidogenesis by luteinized granulosa cells (GCs) and to examine the effect of FSH, E(2), and P on the production of M-CSF by GCs. DESIGN: In vitro experimental study. SETTING: University hospital with an IVF-intracytoplasmic sperm injection program. PATIENT(S): Forty infertile women who underwent intracytoplasmatic sperm injection. INTERVENTION(S): The GCs obtained from these patients during oocyte retrieval were cultured and then treated with different concentrations of M-CSF, FSH, E(2), or P for 72 hours. MAIN OUTCOME MEASURE(S): The M-CSF concentrations were determined by ELISA, and the concentrations of E(2) and P were measured by enzyme immunoassay. RESULT(S): The M-CSF secretion of GCs was enhanced by FSH or E(2) in vitro in a dose-dependent manner but was unaffected by P. On the other hand, M-CSF elicited a production of E(2) and P by GCs in a dose-dependent manner in the presence or absence of FSH. CONCLUSION(S): The results suggest that the growth factor M-CSF might have an important role in ovarian function.