Bacterioneuston control of air-water methane exchange determined with a laboratory gas exchange tank

摘要

[1] The apparent transfer velocities (k_w) of CH_4, N_2O, and SF_6 were determined for gas invasion and evasion in a closed laboratory exchange tank. Tank water (pure Milli-RO water or artificial seawater prepared in Milli-RO) and/or tank air gas compositions were adjusted, with monitoring of subsequent gas transfer by gas chromatography. Derived k_w was converted to “apparent k_(600),” the value for CO_2 in freshwater at 20 ℃. For CH_4, analytical constraints precluded estimating apparent k_(600) based on tank air measurements. In some experiments we added strains of live methanotrophs. In others we added chemically deactivated methanotrophs, non-CH_4 oxidizers (Vibrio), or bacterially associated surfactants, as controls. For all individual controls, apparent k_(600) estimated from CH_4, N_2O, or SF_6 was indistinguishable. However, invasive estimates always exceeded evasive estimates, implying some control of gas invasion by bubbles. Estimates of apparent k600 differed significantly between methanotroph strains, possibly reflecting species-specific surfactant release. For individual strains during gas invasion, apparent k_(600) estimated from CH_4, N_2O, or SF_6 was indistinguishable, whereas during gas evasion, k_(600)-CH_4 was significantly higher than either k_(600)-N_2O or k_(600)-SF_6, which were identical. Hence evasive k_(600)-CH_4/k_(600)-SF_6 was always significantly above unity, whereas invasive k_(600)-CH_4/k_(600)-SF_6 was not significantly different from unity. Similarly, k600-CH4/k_(600)-SF_6 for the controls and k_(600)-N_2O/k_(600)-SF_6 for all experiments did not differ significantly from unity. Our results are consistent with active metabolic control of CH4 exchange by added methanotrophs in the tank microlayer, giving enhancements of ～12 ± 10% for k_(600)-CH_4. Hence reactive trace gas fluxes determined by conventional tracer methods at sea may be in error, prompting a need for detailed study of the role of the sea surface microlayer in gas exchange.