Strong seasonal disequilibrium measured between the oxygen isotope signals of leaf and soil CO exchange

摘要

The oxygen isotope composition (tp#iO) of atmospheric CO is among a very limited number of tools available to constrain estimates of the biospheric gross CO fluxes, photosynthesis and respiration at large scales. However, the accuracy of the partitioning strongly depends on the extent of isotopic disequilibrium between the signals carried by these two gross fluxes. Chamber-based field measurements of total CO and COp#iO fluxes from foliage and soil can help evaluate and refine our models of isotopic fractionation by plants and soils and validate the extent and pattern of isotopic disequilibrium within terrestrial ecosystems. Owing to sampling limitations in the past, such measurements have been very rare and covered only a few days. In this study, we coupled automated branch and soil chambers with tuneable diode laser absorption spectroscopy techniques to continuously capture the tp#iO signals of foliage and soil CO exchange in a Pinus pinaster Act forest in France. Over the growing season, we observed a seasonally persistent isotopic disequilibrium between the tp#iO signatures of net CO fluxes from leaves and soils, except during rain events when the isotopic imbalance became temporarily weaker. Variations in the tp#iO of CO exchanged between leaves, soil and the atmosphere were well explained by theory describing changes in the oxygen isotope composition of ecosystem water pools in response to changes in leaf transpiration and soil evaporation.