PCR Amplification of a Superoxide Dismutase Gene (pasod) from Psychrophilic Bacteria (KOPRI22215) without Genome Information

摘要

Aerobic organisms have adopted delicate defense mechanisms using some antioxidant enzymes, such as superoxide dismutase (SOD) and catalase (CAT) in order to protect themselves from oxidative stress. In most cases, oxidative stresses are caused by a number of reactive oxygen species (ROS) generated as byproducts of respiration. SOD is a ubiquitous metallo-enzyme, detoxifying ROS by catalyzing the dismutation of toxic superoxide anions into O2 and H2O2. In bacteria, four different SODs (Fe-SOD, Mn-SOD, CuZn-SOD, and Ni-SOD) exist with characteristic structural features. For example, SODs from many extremophiles belong to a family of Fe/Mn-SODs, which are composed of an N-terminal domain with a long alpha anti-parallel hairpin and a C-terminal domain with three-stranded antiparallel β-sheets, flanked on both sides by four-a helices according to Pfam hits. Depending on the type of metals, they also have different properties in terms of their oligomeric states as well as selective inhibition.