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A Cre Transgenic Line for Studying V2 Neuronal Lineages and Functions in the Spinal Cord

机译:用于研究V2神经元谱系和脊髓功能的Cre转基因系

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摘要

During spinal neurogenesis, the p2 progenitor domain generates at least two subclasses of interneurons named V2a and V2b, which are components of the locomotor central pattern generator. The winged-helix/forkhead transcription factor Foxn4 is expressed in a subset of p2 progenitors and required for specifying V2b interneurons. Here, we report the generation of a Foxn4-Cre BAC transgenic mouse line that drives Cre recombinase expression mimicking endogenous Foxn4 expression pattern in the developing spinal cord. We used this transgenic line to map neuronal lineages derived from Foxn4-expressing progenitors and found that they gave rise to all neurons of the V2a, V2b, and the newly identified V2c lineages. These data suggest that Foxn4 may be transiently expressed by all p2 progenitors and that the Foxn4-Cre line may serve as a useful genetic tool not only for lineage analysis but also for functional studies of genes and neurons involved in locomotion. genesis 48:667-672, 2010
机译:在脊髓神经发生过程中,p2祖域会生成至少两个中间神经元子类,称为V2a和V2b,它们是运动中心模式发生器的组成部分。有翼螺旋/叉头转录因子Foxn4在p2祖细胞的子集中表达,是指定V2b中间神经元所必需的。在这里,我们报告了Foxn4-Cre BAC转基因小鼠系的产生,该系驱动Cre重组酶表达,模拟在发育中的脊髓中的内源性Foxn4表达模式。我们使用这种转基因系来定位表达Foxn4的祖细胞的神经元谱系,发现它们产生了V2a,V2b和新近鉴定的V2c谱系的所有神经元。这些数据表明,Foxn4可能由所有p2祖细胞瞬时表达,Foxn4-Cre系不仅可以作为谱系分析的有用遗传工具,而且还可以作为运动相关基因和神经元功能研究的有用工具。创世纪48:667-672,2010

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