Pooled Genome-Wide shRNA Sereening: Use of the TRC Library with Lentiviral Knowledge and Expertise Helps Lead to Successful Results

摘要

RNA interference is an effective mechanism for gene silencing whereby double-stranded RNA triggers the cleavage and subsequent degradation of homologous transcript sequences. In this evolutionarily conserved process, longer double-stranded RNA molecules are processed into shorter sequences (21-23 nucleotide small interfering RNAs, or siRNAs) that can bind to the multicomponent RNA-induced silencing complex (RISC). Within this complex, the siRNA is unwound, and the sense strand is cleaved and dissociated. The antisense strand then remains bound and acts as a guide to target activated RISC to complementary mRNA for cleavage and degradation.