Determination of Epinephrine Using Sodium Iodate in Chemiluminescence

摘要

Epinephrine was determined using a lab-made chemiluminescence (CL) system with air pump.Luminol-sodium IO_4~-chemiluminescence system was employed to produce the luminescence of epinephrine.In the reaction,epinephrine was oxidized to produce superoxide or singlet oxygen by periodate in alkaline solution,which enhanced CL of luminol.For optimization,various buffers,such as phosphate,borate,and tris,were studied in this experiment.Compared to NaOH,the phosphate and borate buffer showed better reproducibility with similar sensitivity.Small amount of sample,22 muL,was required for a measurement.The limit of quantification for epinephrine was obtained to be ~10~(-9) g/mL after optimization.